Journal: eLife

Article Title: Simultaneous quantification of mRNA and protein in single cells reveals post-transcriptional effects of genetic variation

doi: 10.7554/eLife.60645

Figure Lengend Snippet: ( A ) Additional copies of the dCAS9-Vp64 gene using a low-copy number (CEN) or a high-copy number (2µ) plasmid affected neither GFP nor mCherry expression. Two replicates per condition are shown as stacked columns of points, each with five points that show the values at the time points at which measurements were taken. ( B ) Additional copies of mCYC1p- mCherry increased red fluorescence in all concentrations of estradiol, but did not extend the linear range of mCherry expression. Three replicates per condition. Cells were grown in SC medium.

Article Snippet: Gene (N.A) , dCAS9 , Addgene 49013 , (doi: 10.1021/sb400081r ) , , .

Techniques: Low Copy Number, Plasmid Preparation, Expressing, Fluorescence

Journal: eLife

Article Title: Simultaneous quantification of mRNA and protein in single cells reveals post-transcriptional effects of genetic variation

doi: 10.7554/eLife.60645

Figure Lengend Snippet: The two plasmids on the top increase the copy number of gRNA binding sites (pCYC1m) and of the mCherry gene. The two plasmids on the bottom increase the copy number of dCAS9-VP64.

Article Snippet: Gene (N.A) , dCAS9 , Addgene 49013 , (doi: 10.1021/sb400081r ) , , .

Techniques: Binding Assay

Journal: eLife

Article Title: Simultaneous quantification of mRNA and protein in single cells reveals post-transcriptional effects of genetic variation

doi: 10.7554/eLife.60645

Figure Lengend Snippet:

Article Snippet: Gene (N.A) , dCAS9 , Addgene 49013 , (doi: 10.1021/sb400081r ) , , .

Techniques: CRISPR, Recombinant, Sequencing, Cloning, Gel Extraction, Isolation, Multiplex Assay, Software, FACS, Flow Cytometry

Journal: Cell Genomics

Article Title: Activation of the imprinted Prader-Willi syndrome locus by CRISPR-based epigenome editing

doi: 10.1016/j.xgen.2025.100770

Figure Lengend Snippet:

Article Snippet: The lentiviral VP64 dCas9 VP64 plasmid (Addgene #232179) was generated by modifying Addgene #59791 to replace GFP with BSD blasticidin resistance.

Techniques: DNA Methylation Assay, Sequencing, Recombinant, Plasmid Preparation, Expressing, Software